abstract：:Retrospective evaluation of the kinetics of cytomegalovirus (CMV) seroconversion with CMV IgM, IgG, and IgG avidity assays, in a Swiss pregnant women population, has shown that the current published CMV serologic diagnostic algorithms were valid and fit for use. In 19% of the cases analyzed, CMV-specific IgM was detec...

journal_title：Diagnostic microbiology and infectious disease

authors： Maine GT,Stricker R,Stricker R

更新日期：2012-07-01 00:00:00

abstract：:During multilocus sequence analysis of Acinetobacter calcoaceticus-Acinetobacter baumannii complex, we identified the evidence of recent genetic recombination between 2 Acinetobacter species. While 3 isolates belonged to A. nosocomialis based on 16S rRNA, gyrB, fusA, gdhB, and rplB gene sequences, they showed close re...

journal_title：Diagnostic microbiology and infectious disease

authors： Kim DH,Park YK,Choi JY,Ko KS

更新日期：2012-07-01 00:00:00

abstract：:Serologic methods are well established for the diagnosis of Mycoplasma pneumoniae infections in humans, but they are less sensitive than polymerase chain reaction (PCR). To improve their sensitivity, a new panel of antigens was tested. Compared with PCR results, up to 92% of PCR-positive patients were confirmed by our...

journal_title：Diagnostic microbiology and infectious disease

authors： Dumke R,Strubel A,Cyncynatus C,Nuyttens H,Herrmann R,Lück C,Jacobs E

更新日期：2012-06-01 00:00:00

abstract：:In order to assess the significance of Candida colonization of intravascular catheters (IVC) in patients without documented candidemia, we retrospectively reviewed all Candida-positive IVC tip cultures over a 4-year period. Cases were defined as those with a culture yielding ≥15 colony-forming units of Candida spp. th...

journal_title：Diagnostic microbiology and infectious disease

authors： López-Medrano F,Fernández-Ruiz M,Origüen J,Belarte-Tornero LC,Carazo-Medina R,Panizo-Mota F,Chaves F,Sanz-Sanz F,San Juan R,Aguado JM

更新日期：2012-06-01 00:00:00

abstract：OBJECTIVES:Bacteremia is a severe bacterial infection with significant mortality and morbidity. Clinical parameters that reliably predict the presence of community-onset bacteremia are less elucidated. METHODS:During 96 randomly selected days between August 2006 and July 2007, a prospective study was conducted to anal...

journal_title：Diagnostic microbiology and infectious disease

authors： Lee CC,Wu CJ,Chi CH,Lee NY,Chen PL,Lee HC,Chang CM,Ko NY,Ko WC

更新日期：2012-06-01 00:00:00

abstract：:This retrospective study aimed to evaluate the clinical, laboratory, and quantitative cerebrospinal fluid (CSF) cryptococcal cell counts for associations with in-hospital outcomes of HIV-infected patients with cryptococcal meningitis. Ninety-eight HIV-infected adult patients with CSF culture-proven cryptococcal mening...

journal_title：Diagnostic microbiology and infectious disease

authors： Vidal JE,Gerhardt J,Peixoto de Miranda EJ,Dauar RF,Oliveira Filho GS,Penalva de Oliveira AC,Boulware DR

更新日期：2012-05-01 00:00:00

abstract：:We compared the performance of 8 Clostridium difficile enzyme immunoassays to cell cytotoxicity neutralization assay and toxigenic culture. The effect of strain type on assay performance was also examined. There were a total of 71 (14.4%) samples in which C. difficile was recovered; 58 (81.7%) of 71 were toxigenic. Co...

journal_title：Diagnostic microbiology and infectious disease

authors： René P,Frenette CP,Schiller I,Dendukuri N,Brassard P,Fenn S,Loo VG

更新日期：2012-05-01 00:00:00

abstract：:Borrelia burgdorferi sensu lato (s. l.), the agent of Lyme disease, is distributed widely worldwide. A large number of polymerase chain reaction (PCR) methods have been developed and used for detection of B. burgdorferi s. l. However, there is a lack of a reference standard because of the genetic diversity of the B. b...

journal_title：Diagnostic microbiology and infectious disease

authors： Yang J,Liu Z,Guan G,Che R,Niu Q,Li Y,Liu J,Ma M,Ren Q,Liu A,Luo J,Yin H

更新日期：2012-05-01 00:00:00

abstract：:Serious Candida glabrata infections, which can be difficult to treat, are often treated with echinocandins. We compared in vitro the effects of high and low concentrations of 3 echinocandins (micafungin [MCF], caspofungin [CAS], and anidulafungin [ANF]), voriconazole (VRC), and amphotericin B (AmB), singly and VRC in ...

journal_title：Diagnostic microbiology and infectious disease

authors： Baltch AL,Lawrence DA,Ritz WJ,Andersen NJ,Bopp LH,Michelsen PB,Carlyn CJ,Smith RP

更新日期：2012-03-01 00:00:00

abstract：:Two automated systems, BD Phoenix and bioMérieux Vitek 2, were compared to a double-disk diffusion method for the detection of inducible resistance to clindamycin in Staphylococcus spp. Analysis of 524 clinical isolates revealed sensitivity and specificity of 100% and 99.6%, respectively, for Phoenix and 91.1% and 99....

journal_title：Diagnostic microbiology and infectious disease

authors： Buchan BW,Anderson NW,Ledeboer NA

更新日期：2012-03-01 00:00:00

abstract：:To evaluate the diagnostic value of immunochromatographic test (ICT) on pleural fluid in diagnosing pneumococcal empyema in children and to determine pneumococcal serotypes, 62 exudative parapneumonic effusions from Korean children were tested with culture, ICT for S. pneumoniae, pneumococcal autolysin polymerase chai...

journal_title：Diagnostic microbiology and infectious disease

authors： Lee JH,Kim SH,Lee J,Choi EH,Lee HJ

更新日期：2012-02-01 00:00:00

abstract：:Pneumocystis jirovecii is an important opportunistic infection in human immunodeficiency virus (HIV)-infected patients. In the developed world, P. jirovecii epidemiology is marked by frequent colonization in immunosuppressed patients, but data on the prevalence of colonization are very limited in sub-Saharan Africa, w...

journal_title：Diagnostic microbiology and infectious disease

authors： Taylor SM,Meshnick SR,Worodria W,Andama A,Davis JL,Cattamanchi A,den Boon S,Yoo SD,Goodman CD,Huang L,International HIV-associated Opportunistic Pneumonias (IHOP) Study.

更新日期：2012-02-01 00:00:00

abstract：:The aim of this study was to assess the phenotypic and genotypic diversity of 56 Arcanobacterium haemolyticum isolates isolated from 51 patients attending primary health care centres and emergency units in the health area of Santander (Cantabria, northern Spain). Phenotypic characterization was based on morphological,...

journal_title：Diagnostic microbiology and infectious disease

authors： García-de-la-Fuente C,Ruiz De Alegría C,Cano ME,Campo AB,Unda F,Agüero J,Martínez-Martínez L

更新日期：2012-01-01 00:00:00

abstract：:Fusarium is an opportunistic fungal pathogen which is emerging as a significant cause of morbidity and mortality in immunocompromised hosts. We present a rare case of F. verticillioides fungemia that occurred in a patient who underwent a second orthotopic liver transplantation for chronic rejection and completely resp...

journal_title：Diagnostic microbiology and infectious disease

authors： Cocchi S,Codeluppi M,Venturelli C,Bedini A,Grottola A,Gennari W,Cavrini F,Di Benedetto F,De Ruvo N,Rumpianesi F,Gerunda GE,Guaraldi G

更新日期：2011-12-01 00:00:00

abstract：:Mycobacterium wolinskyi is an uncommonly encountered rapidly growing mycobacterium. To date, only 12 clinical cases have been reported in the literature. In this report, we describe 5 additional cases of M. wolinskyi infection seen at the Mayo Clinic in Rochester, MN, since 2009. The clinical manifestations were stern...

journal_title：Diagnostic microbiology and infectious disease

authors： Ariza-Heredia EJ,Dababneh AS,Wilhelm MP,Wengenack NL,Razonable RR,Wilson JW

更新日期：2011-12-01 00:00:00

abstract：:Interferon gamma release assays are used for diagnosing latent tuberculosis (TB); however, their role in diagnosing tuberculous pleural effusion (TPE) is not defined. The aim of this study was to evaluate the usefulness of the QuantiFERON-TB Gold In-Tube assay (QFT-IT) and compare this assay with the tuberculin skin t...

journal_title：Diagnostic microbiology and infectious disease

authors： Chung JH,Han CH,Kim CJ,Lee SM

更新日期：2011-11-01 00:00:00

abstract：:Beta-lactam-resistant Enterobacteriaceae represent an important public health problem; however, questions exist about their prevalence and the impact of recent breakpoint changes on clinical practice. We surveyed infectious disease physicians to better understand these issues. Many reported encountering resistant Ente...

journal_title：Diagnostic microbiology and infectious disease

authors： Kallen AJ,Beekmann SE,Limbago B,Lentnek AL,Polgreen PM,Patel J,Srinivasan A

更新日期：2011-11-01 00:00:00

abstract：:Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry (MALDI-TOF-ICMS) was used to differentiate pathogenic Candida species, difficult to identify by traditional methods such as growth and biochemical reactions. Results showed that species complexes like C. parapsilosis, C. orthopsil...

journal_title：Diagnostic microbiology and infectious disease

authors： Santos C,Lima N,Sampaio P,Pais C

更新日期：2011-11-01 00:00:00

abstract：:We aimed to compare the positivity of the QuantiFERON TB gold in-tube (QFT-IT antigens) specific interferon gamma (IFN-γ/QFT-IT) and IFN-γ-inducible protein-10 (IP-10/QFT-IT) assays with tuberculin skin test (TST) among human immunodeficiency virus (HIV)-infected individuals in a TB endemic setting. A total of 180 HIV...

journal_title：Diagnostic microbiology and infectious disease

authors： Kabeer BS,Sikhamani R,Raja A

更新日期：2011-11-01 00:00:00

abstract：:Three polymerase chain reaction (PCR) assays - kinetoplast DNA (kDNA) PCR, 7SL PCR, ITS1 PCR - were compared for their ability to detect leishmanial parasites in the skin tissue aspirates of 212 Palestinian suspect cases of cutaneous leishmaniasis (CL). The kDNA PCR was the most sensitive, detecting 156/170 (91.8%) of...

journal_title：Diagnostic microbiology and infectious disease

authors： Azmi K,Nasereddin A,Ereqat S,Schnur L,Schonian G,Abdeen Z

更新日期：2011-10-01 00:00:00

abstract：:Pneumocystis jirovecii has been detected in lung tissue from patients with chronic obstructive pulmonary disease (COPD) and is associated with disease severity. The regional distribution of the organism in lungs is unknown, but differences in distribution of Pneumocystis could affect estimates of colonization prevalen...

journal_title：Diagnostic microbiology and infectious disease

authors： Sivam S,Sciurba FC,Lucht LA,Zhang Y,Duncan SR,Norris KA,Morris A

更新日期：2011-09-01 00:00:00

abstract：:Polymerase chain reaction (PCR) offers promise in pneumococcal diagnostics, but whether nasopharyngeal carriage causes false-positive results in people colonized with Streptococcus pneumoniae is unknown. We found in serum no positive samples for pneumococcal DNA in 100 carriers and noncarriers using 2 different real-t...

journal_title：Diagnostic microbiology and infectious disease

authors： Rouphael N,Steyn S,Bangert M,Sampson JS,Adrian P,Madhi SA,Klugman KP,Ades EW

更新日期：2011-08-01 00:00:00

abstract：:Phoma species are primarily phytopathogens which have been reported to sporadically cause human disease. We report a patient with phaeohyphomycotic cysts caused by Phoma species, which were initially mistaken for ganglions. ...

journal_title：Diagnostic microbiology and infectious disease

authors： Vasoo S,Yong LK,Sultania-Dudani P,Scorza ML,Sekosan M,Beavis KG,Huhn GD

更新日期：2011-08-01 00:00:00

abstract：:To compare the identification accuracies of VITEK 2 (bioMérieux), MicroScan (Siemens Healthcare), and Phoenix (Becton Dickinson), microbial identification was performed on 160 clinical isolates and 50 reference strains on each of these 3 systems, using the appropriate identification kit provided by each system. Of the...

journal_title：Diagnostic microbiology and infectious disease

authors： Jin WY,Jang SJ,Lee MJ,Park G,Kim MJ,Kook JK,Kim DM,Moon DS,Park YJ

更新日期：2011-08-01 00:00:00

abstract：:Bartonella quintana, known to cause various clinical symptoms, is increasingly recognized as one important cause of culture-negative endocarditis. We report a case of infectious endocarditis with B. quintana on the prosthetic valve, accompanied by proteinase 3-antineutrophil cytoplasmic antibody-positive collagen vasc...

journal_title：Diagnostic microbiology and infectious disease

authors： Yamada Y,Ohkusu K,Yanagihara M,Tsuneoka H,Ezaki T,Tsuboi J,Okabayashi H,Suwabe A

更新日期：2011-07-01 00:00:00

abstract：:The constitutive or inducible macrolide-lincosamide-streptogramin (MLS) phenotype of 30 erm(B)-positive Streptococcus pyogenes isolates was determined by different methods and under various growth conditions and correlated to the sequence of the 5'-untranslated regions of erm(B). The MLS phenotype of one-third of the ...

journal_title：Diagnostic microbiology and infectious disease

authors： Rombini S,Petrelli D,Bolli E,Tran CN,Falconi M,Di Luca MC,Prenna M,Ripa S,Vitali LA

更新日期：2011-07-01 00:00:00

abstract：:Limited information is available on the risk factors for false-negative results with the new generation of QuantiFERON-TB Gold In-Tube (QFT-GIT) tests in non-HIV-infected patients with tuberculosis (TB). We sought to identify risk factors for false-negative QFT-GIT results in culture-confirmed TB patients. We reviewed...

journal_title：Diagnostic microbiology and infectious disease

authors： Kim EY,Park MS,Kim YS,Kim SK,Chang J,Kang YA

更新日期：2011-07-01 00:00:00

abstract：:Fusobacterium nucleatum bacteremia is critical and not well defined. To identify the clinical characteristics and outcomes, we conducted a retrospective review of hospitalized patients from January 2004 to December 2009 at a tertiary center in northern Taiwan. Fifty-seven patients were enrolled. The mean age was 58.1 ...

journal_title：Diagnostic microbiology and infectious disease

authors： Yang CC,Ye JJ,Hsu PC,Chang HJ,Cheng CW,Leu HS,Chiang PC,Lee MH

更新日期：2011-06-01 00:00:00

abstract：:For 244 patients with bacteremia due to extended-spectrum β-lactamase (ESBL)-producing Escherichia coli or Klebsiella pneumoniae treated by ertapenem (73, 29.9%) or either imipenem or meropenem (171, 70.1%), the therapeutic efficacy was evaluated. Ertapenem therapy was effective for patients with ESBL-producing E. col...

journal_title：Diagnostic microbiology and infectious disease

authors： Lee NY,Huang WH,Tsui KC,Hsueh PR,Ko WC

更新日期：2011-05-01 00:00:00

abstract：:Numerous studies have been carried out to determine whether an Epstein-Barr virus (EBV) infection can be considered a risk factor for multiple sclerosis (MS), following the evidence of an increase in IgG response to nuclear antigen-1 (EBNA-1) in both serum and cerebrospinal fluid (CSF) from MS patients. However, the p...

journal_title：Diagnostic microbiology and infectious disease

authors： Villegas E,Santiago O,Carrillo JA,Sorlózano A,Guerrero M,Fernández O,Gutiérrez J

更新日期：2011-05-01 00:00:00

abstract：:Dermatophytic mycetoma is an extremely rare subcutaneous mycosis. Here, we report the case of a 6-year-old girl with clinical, histologic, and mycologic findings consistent with a mycetoma of the scalp caused by Microsporum canis. To our knowledge, this is the first report showing the immunologic and immunogenetic fea...

journal_title：Diagnostic microbiology and infectious disease

authors： Chiapello LS,Dib MD,Nuncira CT,Nardelli L,Vullo C,Collino C,Abiega C,Cortes PR,Spesso MF,Masih DT

更新日期：2011-05-01 00:00:00

abstract：:The emergence of the pandemic influenza virus A H1N1 has made fast and accurate diagnosis essential. However, few well-validated diagnostic techniques exist. The real-time RT-PCR developed by the Centers for Disease Control and Prevention (CDC) is the recommended technique. Our objective was to compare the CDC real-ti...

journal_title：Diagnostic microbiology and infectious disease

authors： López Roa P,Catalán P,Giannella M,García de Viedma D,Sandonis V,Bouza E

更新日期：2011-04-01 00:00:00

abstract：:Beginning on April 2007, a prospective multicenter study was performed to investigate prevalence and epidemiology of microbial pathogens causing bloodstream infections (BSIs). Twenty microbiology laboratories participated to the survey over a 1-year period. A total of 11,638 episodes of BSI occurred in 11 202 patients...

journal_title：Diagnostic microbiology and infectious disease

authors： Luzzaro F,Ortisi G,Larosa M,Drago M,Brigante G,Gesu G

更新日期：2011-04-01 00:00:00

abstract：:This study assessed the demographics, antimicrobial susceptibility, and molecular epidemiology of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) and health care-associated MRSA (HA-MRSA) in Canadian hospitals between 2007 and 2009. Among 3589 S. aureus, 889 (24.8%) were MRSA; 224 (25.2%) we...

journal_title：Diagnostic microbiology and infectious disease

authors： Nichol KA,Adam HJ,Hussain Z,Mulvey MR,McCracken M,Mataseje LF,Thompson K,Kost S,Lagacé-Wiens PR,Hoban DJ,Zhanel GG,Canadian Antimicrobial Resistance Alliance (CARA).

更新日期：2011-03-01 00:00:00

abstract：:We report on a scrub typhus patient who contracted meningoencephalitis during doxycycline administration. This is the first case of scrub typhus in which doxycycline concentrations were measured in serum and cerebrospinal fluid. Clinicians should be alerted to the possibility that meningoencephalitis can occur because...

journal_title：Diagnostic microbiology and infectious disease

authors： Kim DM,Kim YS,Cho HY,Lee YB

更新日期：2011-03-01 00:00:00

abstract：:bla(OXA-23) and bla(OXA-66) were detected in 49 Acinetobacter baumannii isolates. These isolates were assigned to 7 subtypes by enterobacterial repetitive intergenic consensus polymerase chain reaction and 7 representative isolates were of ST92 or ST75. In most cases, the ISAba1-bla(OXA-23)-ATPaseΔ structure was ident...

journal_title：Diagnostic microbiology and infectious disease

authors： Wang X,Zong Z,Lü X

更新日期：2011-02-01 00:00:00

abstract：:During the early stages of the 2009/2010 swine-origin H1N1 influenza A (S-OIV H1N1 FluA) outbreak, the development and validation of sensitive and specific detection methods were a priority for rapid and accurate diagnosis. Between May and June 2009, 2 real-time reverse transcriptase-polymerase chain reaction (rRT-PCR...

journal_title：Diagnostic microbiology and infectious disease

authors： Duncan C,Guthrie JL,Tijet N,Elgngihy N,Turenne C,Seah C,Lau R,McTaggart L,Mallo G,Perusini S,Rebbapragada A,Melano R,Low DE,Farrell D,Guyard C

更新日期：2011-02-01 00:00:00

abstract：:Carbapenem susceptibility in Enterobacteriaceae (M9921, M9959) revealed the presence of MBLs bla(VIM-2) (M9959) and bla(IMP-8) (M9921), both as first cassettes of class-1-integrons. ESBL bla(PER-2) was detected in both strains and M9921 also harboured qnrB10, aac(6')-Ib and aac(6')-Ib-cr. This is the first report of M...

journal_title：Diagnostic microbiology and infectious disease

authors： Gomez S,Rapoport M,Togneri A,Viegas-Caetano J,Faccone D,Corso A,Petroni A,Pasteran F

更新日期：2011-01-01 00:00:00

abstract：:Thermostable direct hemolysin encoded by tdh gene has been considered an important virulence factor in pathogenic Vibrio parahaemolyticus. Two-step ultrarapid real-time polymerase chain reaction (URRT PCR) with a microchip was devised to detect V. parahaemolyticus carrying tdh gene. This novel method has a 6-μL reacti...

journal_title：Diagnostic microbiology and infectious disease

authors： Kang MH,Kim IW,Lee DW,Yoo MS,Han SH,Yoon BS

更新日期：2011-01-01 00:00:00

abstract：:This study evaluates 3 selective media (Pylori agar [bioMérieux, France], BD Helicobacter agar, modified [Becton Dickinson, USA], and an in-house medium) designed for Helicobacter pylori isolation. Ninety-eight strains were isolated from 400 gastric biopsies. The media were equally efficient for Helicobacter pylori's ...

journal_title：Diagnostic microbiology and infectious disease

authors： Miendje Deyi VY,Van den Borre C,Fontaine V

更新日期：2010-12-01 00:00:00